Composite

Part:BBa_K2072000:Design

Designed by: ching song   Group: iGEM16_UST_Beijing   (2016-10-04)


pBAD-driven synthetic beta-glucosidase gene with araC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1868
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1101
    Illegal AgeI site found at 2033
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 170
    Illegal SapI.rc site found at 605
    Illegal SapI.rc site found at 2050


Design Notes

The enzyme is to be used for preparation of notoginseng root extract for ginsenosides bio-availability improvement.


Source

The beta-glucosidase gene is a truncated form of a natural gene from Microbacterium esteraromaticum strain GS514 (GenBank: JN603821.1) with optimized codons according to E.coli codon bias. The araC coding sequence and bi-directional promoter pBAD/araC are from E.coli K12 genome.

References

Quan,L.H., Min,J.W., Jin,Y., Wang,C., Kim,Y.J. and Yang,D.C. Enzymatic biotransformation of ginsenoside Rb1 to compound K by recombinant beta-glucosidase from Microbacterium esteraromaticum. J. Agric. Food Chem. 60 (14), 3776-3781 (2012)